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GRAPHENE OXIDE 'DISCOVERED' IN PFIZER VACCINE BY DR CAMPRA - THE ALMERIA PAPER - ENG TRANSLATION

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OFFICIAL INTERIM REPORT IN ENGLISH (UNIVERSITY OF ALMERIA)
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EXTRACT:

Dr Campra


Background

• Mr. Ricardo Delgado Martín requests PROVISION OF RESEARCH SERVICES to the UAL

named:

"DETECTION OF GRAPHENE IN AQUEOUS SUSPENSION SAMPLE"

- On 06/10/2021 1 vial was received by courier, labeled with the following text:

- “COMIRNATY™ .Sterile concentrate. COVID-19 mRNA. 6 doses after

dilution.

- Discard date / time: PAA165994.LOT / EXP: EY3014 08/2021 ”

- Origin and traceability: unknown

- State of conservation: refrigerated

- Maintenance during the study: refrigerated

- Coding of the problem sample to be analyzed: RD1

Preliminary observations of the test sample RD1

Description:

- Sealed vial, with rubber and aluminum cap intact, of 2 ml capacity,

containing a 0.45 ml cloudy aqueous suspension.

- RNA extraction and quantification is performed

- Presence of uncharacterized nanometric microbiology is observed, visible at 600X in optical microscope


Sample processing

1. Dilution in 0.9% sterile physiological saline (0.45 ml + 1.2 ml)


2. Polarity fractionation: 1.2 ml hexane + 120 ul of RD1 sample


3. Extraction of hydrophilic phase


4. Extraction and quantification of RNA in the sample


5. Electron and optical microscopy of aqueous phase


Preliminary analysis: extraction and quantification of Rna in the sample

1. RNA extraction: Kit https://www.fishersci.es/shop/products/ambion-purelink-rna-mini-kit-

7/10307963


2. Quantification of total UV absorbance in spectrophotometer


3. Specific quantification of Rna by fluorescence QUBIT2.0:


UV absorption spectrum of the aqueous phase of the RD1 sample (Nano-

drop team)

Maximum absorption of SAMPLE RD1 (260-270 nm)


- RNA. It presents usual maximums at 260 nm. Total concentration estimated by QUBIT2.0

fluorometry: 6 ng / ul


- The spectrum reveals the presence of a high quantity of substances or substances other than Rna

with maximum absorption in the

same region, with a total estimated at 747 ng / ul (uncalibrated estimate)


- Reduced graphene oxide (RGO) has absorption maxima at 270 nm, compatible with the spectrum

obtained (Thema et al, 2013. Journal of Chemistry ID 150536)


- The maximum absorption obtained DOES NOT ALLOW TO DISCARD the presence of

graphene in the sample. The minimum amount of RNA detected by QUBIT2.0 only explains a residual percentage of the total UV absorption of the sample.


OBJECTIVE: Microscopic identification of graphene derivatives

METHODOLOGY:

1. Imaging in optical and electron microscopy

2. Comparison with literature images and reduced graphene oxide standard sample


TRANSMISSION ELECTRON MICROSCOPY (TEM)

Electron microscope JEM-2100Plus

Voltage: 200 kV

Resolution 0.14 nm

Magnification up to x1,200,000

2


TRANSMISSION ELECTRON MICROSCOPY (TEM)

Electron microscopy (TEM) is commonly used to image graphene nanomaterials. It has become a fairly standard and easy to use an instrument that is capable of imaging individual layered graphene

sheets.

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