GRAPHENE OXIDE 'DISCOVERED' IN PFIZER VACCINE BY DR CAMPRA - THE ALMERIA PAPER - ENG TRANSLATION

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EXTRACT:

Dr Campra

Background

• Mr. Ricardo Delgado Martín requests PROVISION OF RESEARCH SERVICES to the UAL

named:

"DETECTION OF GRAPHENE IN AQUEOUS SUSPENSION SAMPLE"

- On 06/10/2021 1 vial was received by courier, labeled with the following text:

- “COMIRNATY™ .Sterile concentrate. COVID-19 mRNA. 6 doses after

dilution.

- Discard date / time: PAA165994.LOT / EXP: EY3014 08/2021 ”

- Origin and traceability: unknown

- State of conservation: refrigerated

- Maintenance during the study: refrigerated

- Coding of the problem sample to be analyzed: RD1

Preliminary observations of the test sample RD1

Description:

- Sealed vial, with rubber and aluminum cap intact, of 2 ml capacity,

containing a 0.45 ml cloudy aqueous suspension.

- RNA extraction and quantification is performed

- Presence of uncharacterized nanometric microbiology is observed, visible at 600X in optical microscope

Sample processing

1. Dilution in 0.9% sterile physiological saline (0.45 ml + 1.2 ml)

2. Polarity fractionation: 1.2 ml hexane + 120 ul of RD1 sample

3. Extraction of hydrophilic phase

4. Extraction and quantification of RNA in the sample

5. Electron and optical microscopy of aqueous phase

Preliminary analysis: extraction and quantification of Rna in the sample

1. RNA extraction: Kit https://www.fishersci.es/shop/products/ambion-purelink-rna-mini-kit-

7/10307963

2. Quantification of total UV absorbance in spectrophotometer

NanoDrop™ https://www.thermofisher.com/order/catalog/product/ND-2000#/ND-2000

3. Specific quantification of Rna by fluorescence QUBIT2.0:

https://www.thermofisher.com/es/es/home/references/newsletters-and-journals/bioprobes-journal-of-cell-biology-applications/bioprobes-issues-2011/bioprobes-64-april-2011/the-qubit-2-0-fluorometer-april- 2011.html

UV absorption spectrum of the aqueous phase of the RD1 sample (Nano-

drop team)

Maximum absorption of SAMPLE RD1 (260-270 nm)

- RNA. It presents usual maximums at 260 nm. Total concentration estimated by QUBIT2.0

fluorometry: 6 ng / ul

- The spectrum reveals the presence of a high quantity of substances or substances other than Rna

with maximum absorption in the

same region, with a total estimated at 747 ng / ul (uncalibrated estimate)

- Reduced graphene oxide (RGO) has absorption maxima at 270 nm, compatible with the spectrum

obtained (Thema et al, 2013. Journal of Chemistry ID 150536)

- The maximum absorption obtained DOES NOT ALLOW TO DISCARD the presence of

graphene in the sample. The minimum amount of RNA detected by QUBIT2.0 only explains a residual percentage of the total UV absorption of the sample.

OBJECTIVE: Microscopic identification of graphene derivatives

METHODOLOGY:

TRANSMISSION ELECTRON MICROSCOPY (TEM)

Electron microscope JEM-2100Plus

Voltage: 200 kV

Resolution 0.14 nm

Magnification up to x1,200,000

2

TRANSMISSION ELECTRON MICROSCOPY (TEM)

Electron microscopy (TEM) is commonly used to image graphene nanomaterials. It has become a fairly standard and easy to use an instrument that is capable of imaging individual layered graphene

sheets.